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1.
Pesqui. vet. bras ; 38(6): 1217-1224, jun. 2018. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-955432

ABSTRACT

Este trabalho visou avaliar os efeitos de sulfato de vincristine sobre os testículos de ratos tratados na fase pré púbere, sobretudo quanto às alterações das células de Sertoli e das células germinativas. Foram utilizados 30 animais controles e 30 tratados com sulfato de vincristine. As aplicações da droga ocorreram aos 15 dias de vida, e a eutanásia aos 40, 64 e 127 dias de vida para possibilitar a avaliação em diferentes estágios de desenvolvimento reprodutivo. Foram realizadas medidas biométricas (pesos corpóreos e testiculares), medidas morfométricas testiculares, (eixos testiculares maiores e menores, diâmetros testiculares de túbulo e lúmen seminíferos, e altura do epitélio seminífero) e estereológicas (volumes testiculares e as densidades de volume do tecido tubular e do tecido intersticial testicular). As medidas biométricas foram feitas em todos os animais do experimento, e as avaliações morfométricas e estereológicas foram realizadas em 200 túbulos seminíferos. Os resultados demonstraram que sulfato de vincristine reduz parâmetros biométricos como peso corpóreo, peso testicular e volume testicular total. Variáveis morfométricas e estereológicas como diâmetro dos túbulos seminíferos, altura do epitélio seminífero e volume dos túbulos seminíferos também foram reduzidos. Os tipos celulares mais atingidos foram as espermatogônias, espermátides tardias e células de Sertoli.(AU)


This study evaluated the vincristine sulfate effect on rat testes treated in pre pubertal stage, especially regarding the changes of Sertoli cells and germ cells. Thirty control rats and 30 rats treated with vincristine sulfate were used. The drug application occurred at 15 days of life, and euthanasia at 40, 64 and 127 days of life to enable evaluation at different stages of reproductive development. Biometric measurements were performed (body and testicular weights), testicular morphometric measures (major and minor testicular axis and of seminiferous tubule and seminiferous lumen) and stereological (testicular volumes and volume densities of the tubular and testicular interstitial tissue). The biometric measurements were made on all rats in the experiment, and morphometric and stereological analysis was carried out in 200 seminiferous tubules. The results demonstrate that vincristine sulfate reduces biometric parameters such as body weight, testicular weight and the total testicular volume. Morphometric and stereological variables as diameter of the seminiferous tubules, height of the seminiferous epithelium and volume of the seminiferous tubules were also reduced. The most affected cell types were spermatogonia, late spermatids and Sertoli cells.(AU)


Subject(s)
Animals , Rats , Rats, Inbred Strains/abnormalities , Vincristine/adverse effects , Testis/abnormalities
2.
Article in English | IMSEAR | ID: sea-152965

ABSTRACT

The crude methanolic extract of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits and its different fractions have been investigated for the evaluation of in vitro cytotoxic potential. The dried and powder fruits were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions. Brine shrimp (Artemia salina) lethality bioassay was used to investigate the cytotoxic potential of A. bilimbi. Compared to vincristine sulfate (with LC50 of 0.839 μg/ml) methanolic extract, carbon tetrachloride and pet-ether soluble fractions demonstrated a significant cytotoxic potential (having LC50 of 0.005μg/ml, 1.198μg/ml and 0.781μg/ml, respectively). The LC50 values of chloroform and aqueous soluble fractions were 5.691μg/ml and 6.123μg/ml, respective-ly. This study reveals that A. bilimbi possesses effective cytotoxic properties and hence can be a potential source for the isolation of active principle(s) for cancer therapy.

3.
Article in English | IMSEAR | ID: sea-167930

ABSTRACT

The crude methanolic extract of Averrhoa bilimbi Linn. (Oxalidiaceae) fruits and its different fractions have been investigated for the evaluation of in vitro cytotoxic potential. The dried and powder fruits were extracted with methanol at room temperature and the concentrated methanolic extract was fractionated by the modified Kupchan partitioning method to provide pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions. Brine shrimp (Artemia salina) lethality bioassay was used to investigate the cytotoxic potential of A. bilimbi. Compared to vincristine sulfate (with LC50 of 0.839 μg/ml) methanolic extract, carbon tetrachloride and pet-ether soluble fractions demonstrated a significant cytotoxic potential (having LC50 of 0.005μg/ml, 1.198μg/ml and 0.781μg/ml, respectively). The LC50 values of chloroform and aqueous soluble fractions were 5.691μg/ml and 6.123μg/ml, respective-ly. This study reveals that A. bilimbi possesses effective cytotoxic properties and hence can be a potential source for the isolation of active principle(s) for cancer therapy.

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